Use of propidium monoazide for live/dead distinction in microbial ecology
Article Abstract:
Four experiments performed to study the usefulness of propidium monoazide (PMA) treatment of mixed bacterial communities comprising intact and compromised cells in combination with end-point PCR. The two experiments suggest that PMA treatment affects banding patterns in denaturing gradient gel electrophoresis community profiles.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples
Article Abstract:
A new concept is developed with the viable/dead stain ethidium monoazide (EMA) in combination with real-timePCR (EMA-PCR). EMA-PCR offers a novel real-time PCR method for quantitative distinction between viable and dead cells with potentially very wide application.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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