In situ detection of bacteria in continuous-flow cultures of seawater sediment suspension with fluorescently labelled rRNA-directed oligonucleotide probes

Article Abstract:

Natural bacterial populations in continuous-flow cultures of seawater sediment suspensions were studied using rRNA targeted and fluorescently labelled oligonucleotide probes. Around 40-80% of the DAPI-stained cells were detected with the EUB338 probe, and a shift in the composition of the natural bacterial population was also detected. The technique provides insights into the structure of populations growing in sediment suspensions.

Usage, Nucleic acid probes

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Bacillus subtilis ORF yybQ encodes a manganese-dependent inorganic pyrophosphatase with distinctive properties: the first of a new class of soluble pyrophosphatase?

Article Abstract:

Soluble inorganic pyrophosphatase is found in most living cells and it acts to remove pyrophosphate (PPi) generated by biosynthetic pathways. The identity of the Bacillus subtilis PPiase with the translation product of an ORF in the B. subtilis genome, has been identified. It appears unrelated to other PPiases but is similar to three other putative prokaryotic proteins. A new class of PPiase enzyme, type C, is proposed.

Bacillus subtilis

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