Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae

Article Abstract:

Metabolic Flux Analysis (MFA) was employed as part of the stoichiometric model for describing the anaerobic metabolism of Saccharomyces cerevisiae during growth in glucose-limited continuous cultures. The model consisted of quantifying the levels of acetate, CO2, ethanol, glucose, glycerol, NH4+, pyruvate, succinate, carbohydrates, DNA, lipids, proteins and RNA. It was found that the accurate measurement of CO2, ethanol, glucose, glycerol and protein was critical for the calculation of the flux distribution.

Usage, Anaerobiosis, Stoichiometry

---

Physiological effects of nitrogen starvation in an anaerobic batch culture of Saccharomyces cerevisiae

Article Abstract:

Nitrogen starvation affects the anaerobic physiology of Saccharomyces cerevisiae, particularly glycerol formation, protein turnover, catabolite inactivation of the sugar-transport system, the cellular composition, the cell cycle and growth energetics. This was gleaned from a study of cells cultivated in a bioreactor, whose nitrogen source were depleted while there was still sufficient glucose in the mineral medium. The cells' growth and product formation were then monitored.

Physiological aspects, Genetic aspects, Physiology, Protein nitrogen

---

High exogenous concentrations of phenoxyacetic acid are crucial for a high penicillin V productivity in Penicillum chrysogenum

Article Abstract:

Glucose was utilized as a growth-limiting component for a high-penicillin-yielding strain of Penicillium chrysogenum grown in a chemically defined medium. In this study, the high production of penicillin was sustained by maintaining a high amount of phenoxyacetic acid concentration in the strain. This also reduced the loss of the pathway intermediate isopenicillin N in the extracellular medium.

Bacterial growth, Penicillin, Penicillins

Similar abstracts:

• Abstracts: Antisense transcription controls cell fate in Saccharomyces cerevisiae. Degradation-mediated protein quality control in the nucleus
• Abstracts: Metabolic fluxes in chemostat cultures of Schizosaccharomyces pombe grown on mixtures of glucose and ethanol. Coordination of sucrose uptake and respiration in the yeast Debaryomyces yamadae
• Abstracts: Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. Monocentric and polycentric anaerobic fungi produce structurally related cellulases and xylanases
• Abstracts: Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid
• Abstracts: Isolation of the mRNA-capping enzyme and ferric-reductase-related genes from Candida albicans. Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.