Genetic elements of plant viruses as tools for genetic engineering

Article Abstract:

Genomes of plant viruses encode a variety of regulatory sequences which are useful for controlling gene expression in plants. Plant virus genomes encode elements involving control of plant virus nucleic acid synthesis, translational control, posttranslational protein modification and sorting. Bromoviruses, hordeiviruses, tombusviruses and potexviruses are some of the viruses used effectively as epichromosomal expression vectors. However, the possibility of inducing evolutionary shifts in viruses has to be considered before using these control methods on a large scale.

Genomes, Plant genetic engineering

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Satellite RNAs of plant viruses: structures and biological effects

Article Abstract:

Satellite RNAs are usually associated with plant viruses. They rely on their helper viruses for encapsidation and replication. Classification is based on their coding capacity. They can either be the larger or smaller satellites. Smaller satellites containing 194 to 700 nucleotides are subgrouped on the basis of their nucleotide sequence and structure. They do not appear to encode any functional proteins. A functional analysis grouped these satellites based on their structural relatedness on which their biological functions are dependent.

Analysis, RNA, Nucleic acids

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Viral RNA as a potential target for two independent mechanisms of replicase-mediated resistance against cucumber mosaic virus

Article Abstract:

The replicase enzymes of tobacco inhibit the transcription or movement of the cucumber mosaic viruses (CMV) by directly acting on the viral RNA. The binding of replicase to an inactive 2a transgene protein, encoded by the CMV-RNA2 gene, inhibits the formation of a functional complex. In the absence of a functional complex, the CMV is unable to replicate. Replicase also confers resistance by inhibiting the movement of CMV. The accumulation and transcription of viral RNA is unaffected.

Plants, Plants (Organisms), Enzymes, Diseases and pests, Cucumber mosaic virus

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