Genetic instability and strain degeneration in Streptomyces rimosus

Article Abstract:

Streptomyces rimosus R6 exhibits a high level of morphological instability, as reflected in sporulation, pigmentation, colony morphology, oxytetracycline production and oxytetracycline resistance phenotypes. Based on oxytetracycline resistance, three classes of variants were observed. Pulsed-field gel electrophoresis showed that most of these variants exhibit a reproducible large-scale DNA rearrangement, which probably included a deletion and a low-level repetition of a DNA fragment. The implications of these results in strain degeneration and antibiotic overproduction are discussed.

Identification and classification, Mutation (Biology), Mutation, Chromosome deletion, Degeneration, Decadence

---

Physical mapping shows that the unstable oxytetracycline gene cluster of Streptomyces rimosus lies close to one end of the linear chromosome

Article Abstract:

A physical map of the Streptomyces rimosus R6-501 8 Mb linear chromosome and its oxytetracycline (OTC) gene cluster was analyzed based on standard molecular genetic techniques. Analysis of the Streptomyces rimosus R6-501 8 Mb linear chromosome indicated the presence of 550 kb inverted repeats and a possible transposable element that mediated the formation of a long inverted repeat structure. Furthermore, the genetic organization of Streptomyces rimosus was similar to the genome map of Streptomyces coelicolor A3 strains.

Analysis, Chromosomes, Microbial genetics

---

Genetics of Streptomyces rimosus, the oxytetracycline producer

Article Abstract:

Genetics of Streptomyces rimosus, the producer of oxytetracyclin killer, is investigated. The integrated genomic data provided by the study presents a platform to undertake their manipulation of the S. rimosus chromosome, informed by observations from other species, which increase antibiotic productivity, in a well-defined way.

Science & research, Thailand, Antibiotics, Bacterial genetics

Similar abstracts:

• Abstracts: Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae. Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae
• Abstracts: Mineralization of sulfonated azo dyes and sulfanilic acid by Phanerochaete chrysosporium and Streptomyces chromofuscus
• Abstracts: Contacts in context: promoter specificity and macromolecular interactions in transcription. Transcript cleavage factors from E. coli
• Abstracts: Transduction, restriction and recombination patterns in Escherichia coli. Sequence comparisons
• Abstracts: Use of an exotic carbon source to selectively increase metabolic activity and growth of Pseudomonas putida in soil

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.