Use of an exotic carbon source to selectively increase metabolic activity and growth of Pseudomonas putida in soil
Article Abstract:
The growth and respiration of Pseudomonas putida PpG7 was monitored in three field soils supplemented with the exotic carbon source, salicylate. Based on evolved carbon dioxide, the results showed that population density and activity are not neccessarily correlated. The addition of salicylate could selectively increase the growth and metabolism of PpG7, but the increase in growth was not concomitant with increase in activity. These results emphasize the importance of using an ecological kinetic approach when studying the population dynamics of a particular organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Enhanced growth and activity of a biocontrol bacterium genetically engineered to utilize salicylate
Article Abstract:
Pseudomonas putida R20 is a beneficial organism capable of reducing sugar beet seed rot by Pythium ultimum. Plasmid NAH7, which encodes enzymes for naphthalene and salicylate degradation, was transferred to R20 to determine if the engineered bacterium could be grown selectively in agricultural field soil amended with salicylate. The engineered bacterium showed excellent viability, stability and expression of NAH7. These results demonstrate that biological control organisms can be enhanced by the introduction of specific metabolic traits.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Enhancement of population densities of Pseudomonas putida PpG7 in agricultural ecosystems by selective feeding with the carbon source salicylate
Article Abstract:
The effects of salicylate amendment and soil fumigation on the growth of Pseudomonas putida PpG7 introduced in agricultural field soils planted with tomato were investigated. The results showed that soil fumigation could significantly increase the population densities of PpG7. Other factors that affected population densities were inoculum level, field site and soil depth. Salicylate selectively increased PpG7 levels, and coupled with soil fumigation, caused a significant reduction in population densities of fungal salicylate users.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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