HIV-1 cDNA integration: requirement of HMG I(Y) protein for function of preintegration complexes in vitro

Article Abstract:

In vitro experiments were carried out to study the role of the high mobility group protein, HMG I(Y), in processes occurring within cells that are infected with human immunodeficiency virus type 1 (HIV-1). HMG I(Y) has previously been identified as a nonhistone chromosomal protein that is important for transcriptional control and chromosomal architecture. The results revealed that HMG I(Y) is required for the DNA-cutting and -joining reactions which are essential steps in DNA integration, a function of preintegration complexes (PICs).

Usage, Cell culture, Tissue culture

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HIV accessory proteins: leading roles for the supporting cast

Article Abstract:

Nef, Vpu, Vif and Vpr-Vpx are significant factors in HIV malignancy. These are accessory only in name. These factors are significant targets for the treatment of HIV-caused disease. Those who think of ways of dealing with these proteins will need to understand three factors very well: how the proteins act, their interlocutors within the cell, their three-dimensional structure. In vitro assays will be useful in showing their activities.

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Nef-induced CD4 degradation: a diacidic-based motif in Nef functions as a lysosomal targeting signal through the binding of beta-COP in endosomes

Article Abstract:

A study was conducted to show that the Nef protein targets internalized CD4 molecules for degradation by functioning as a connector between CD4 and the beta subunit of COPI coatomers in endosomes. Total bacterial extracts were produced and purified on glutathione beads. Beta-COP was then determined as its own downstream partner. Moreover, a new category of endosomal sorting motif was identified.

Cell membranes, Plasma membranes, CD4 lymphocytes

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