Homofermentive lactate production cannot sustain anaerobic growth of engineered saccharomyces cerevisiae: Possible consequence of energy-dependent lactate export
Article Abstract:
Saccharomyces cerevisiae is engineered to homofermentative lactate-producing yeast through deletion of the three genes encoding pyruvate decarboxylase-negative S.cerevisiae strains, the engineered strain required small amounts of acetate for the synthesis of cytosolic acetyl-coenzyme. The conclusion states that the absence of net ATP production is probably due to a metabolic energy requirement for lactate export.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Physiological characterization of the ARO10-dependent, broad-substrate-specificity 2-oxo acid decarboxylase activity of Saccharomyces cerevisiae
Article Abstract:
A study was conducted to analyze the substrate specificity of the ARO10-dependant decarboxylase activity in Saccharomyces cerevisiae, and its impact on the production of fusel alcohols and acids. The studies conducted indicate the involvement of post-transcriptional regulation and a second protein in the ARO10-dependent, broad-substrate-specificity decarboxylse activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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