Identification of procaryotic repetitive DNA suitable for use as fingerprinting probes
Article Abstract:
A method which enables the rapid identification of procaryotic repetitive sequences for use as DNA fingerprinting probes was developed. The technique involves shotgun cloning of restricted genomic DNA and selection of clones containing repetitive DNA. Confirmation that the clones contained repeated sequences was by Southern hybridization. The sequences were used for specific and sensitive detection of bacteria and as target sequences for integration of reporter genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences
Article Abstract:
A new bacterial identification protocol involving fluorescent labeling and polymerase chain reaction has been developed. The protocol, which simulates hybridization between DNA sequences, was used to examine the SSU rRNA sequences in the Ribosomal Database Project bank. Analysis suggested that length differences in terminal restriction fragments of polymerase chain reaction products from the SSU rRNA sequences can pinpoint several bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Differences in the hybridization pattern of Bacillus subtilis genes coding for rRNA depend on the method of DNA preparation
Article Abstract:
DNA was prepared from Bacillus subtilis by organic deproteinization, potassium acetate deproteinization and the utilization of cetyltrimethylammonium bromide. Hybridization with an rDNA probe done afterwards reveled conflicting patterns of gene coding. Results indicate that the presence of peptidoglycan in the purified B. subtilis strain restricted access to the DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The evolution of tandemly repetitive DNA: recombination rules. Active mutator elements suppress the knotted phenotype and increase recombination at the Kn1-0 tandem duplication
- Abstracts: The evolution of tandemly repetitive DNA: recombination rules. part 2 Molecular analysis of lambda bio transducing phage produced by oxolinic acid-induced illegitimate recombination in vivo
- Abstracts: Degradation of a sodium acrylate oligomer by an Arthrobacter sp. Biodegradability and mechanical prooperties of poly-(beta-hydroxybutyrate-Co-beta-hydroxyvalerate)-starch blends
- Abstracts: Influence of two plant species (flax and tomato) on the distribution of nitrogen dissimilative abilities within fluorescent Pseudomonas spp
- Abstracts: Abundance of virus-sized non-DNase-digestible DNA (coated DNA) in eutrophic seawater. High-temperature fluorescent in situ hybridization for detecting Escherichia coli in seawater samples, using rRNA-targeted oligonucleotide probes and flow cytometry