Identification of type A, B, E, and F botulinum neurotoxin genes and of botulinum neurotoxigenic clostridia by denaturing high-performance liquid chromatography
Article Abstract:
Denaturing high-performance liquid chromatography (DHPLC) is a new method for speedy viewing of nucleotide polymorphisms in PCR products, and this method is utilized to check the type A, B, E, and F botulinum neurotoxin genes. Distinct reports were procured for each genus and species, indicating that this method would be a dependable alternative to the progression of speedy detection of food borne pathogens.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Biosensor detection of botulinum toxoid A and staphylococcal enterotoxin B in food
Article Abstract:
Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Characterization of neutralizing antibodies and identification of neutralizing epitope mimics on the Clostridium botulinum neurotoxin type A
Article Abstract:
Researchers used the phage display method to identify the B-cell epitopes of two monoclonal antibodies against Clostridium botulinum neurotoxin type A. This information could be useful in designing a vaccine against botulism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers
- Abstracts: Aerobic denitrification of Pseudomonas aeruginosa monitored by online NAD(P)H fluorescence. The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa
- Abstracts: Thermal tolerance of Mycobacterium paratuberculosis. Persistence of Mycobacterium parabacterium during manufacture and ripening of Cheddar cheese
- Abstracts: Characterization of Salmonella enterica serovar typhimurium from marine environments in coastal waters of Galicia (Spain)
- Abstracts: A computer-simulated restriction fragment length polymorphism analysis of bacterial small-subunit rRNA genes: efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature