Immunomagnetic capture PCR for rapid concentration and detection of hepatitis A virus from environmental samples
Article Abstract:
Immunomagnetic capture reverse transcription polymerase chain reaction (PCR) was used to investigate the concentration of hepatitis A virus (HAV) from environmental samples by membrane filter-based urea-arginine phosphate buffer. Results revealed that IC PCR is sensitive enough to detect small amounts of cell culture-adapted HAV in sewage samples and inoculated water. IC PCR was specific and did not yield positive reactions with poliovirus 1, HAV RNA or bacteriophages. A field trial showed that 2 of 18 sewage samples tested by IC PCR were positive for HAV.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples
Article Abstract:
A new method of detecting viable Cryptosporidium parvum oocysts by immunomagnetic capture polymerase chain reaction (IC-PCR) is presented. IC-PCR is sensitive enough to detect the presence of Cryptosporidium oocysts in drinking water. It is not as time-consuming and as labor-intensive over existing methods of detecting oocysts since it does not use membrane filters. There is also no need to remove entrapped oocysts or to perform fluorescent-antibody staining.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique
Article Abstract:
A simplified version of the randomly amplified polymorphic DNA technique was used to genetically characterize Cryptosporidium parvum. This approach involved the introduction of a genomic DNA isolation method that used in vitro excystation and specific immunomagnetic separation of Cryptosporidium parvum oocysts. Results indicated that it was effective in isolating an estuary C. parvum isolate from several bovine isolates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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