In situ classification and image cytometry of pelagic bacteria from a high mountain lake (Gossenkollesse, Austria)

Article Abstract:

A research was conducted to investigate cell sizes of pelagic bacteria after determinative hybridization with rRNA-targeted labeled oligonucleotide probes using a procedure based on established image analysis techniques modified for objects simultaneously stained with two fluorescent dyes. The LUCIA D computer software was utilized to record images while the numbers of bacteria that hybridized with different probes were calculated from total bacterial abundance counts. Results suggested that a class of large beta subclass proteobacteria disappeared during the ice break period.

Microbial ecology, Nucleic acid probes, Ribosomal RNA, Bacteriology, Bacterial cultures

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Suitability of flow cytometry for estimating bacterial biovolume in natural plankton samples: comparison with microscopy data

Article Abstract:

Bacterioplankton samples from 80 lakes are used to assess the relationship between flow cytometry data and epifluorescence microscopy measurements and to estimate bacterial biovolume and cell size distribution. It is indicated that flow cytometry is used to properly estimate bacterioplankton biovolume with accuracy similar to those of more time-consuming microscopy methods.

Genetic aspects, Flow cytometry, Plankton

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Catalyzed reported deposition-fluorescence in situ hybridization protocol to evaluate phagotrophy in mixotrophic protists

Article Abstract:

A catalyzed reported deposition-fluorescence in situ hybridization protocol particularly suited to assess the phagotrophy of mixotrophic protists on prokaryotes is described. The protocol allows to detect almost 100% of planktonic prokaryotes and to demonstrate archaeal cells ingested by mixotrophic protists.

Prokaryotes, Archaeabacteria, Hybridization, Archaea

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