In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture
Article Abstract:
A mixed anaerobic bacterial culture was employed in the investigation of the reductive dehalogenation of vinyl chloride. The rate was measured through the formation of ethene which increased exponentially. Trichloroethene was a good substrate for reductive dehalogenation while tetrachloroethene is a poor substitute. The dehalogenating activity was surmised to be a membrane function through an in vitro assay using cell extracts.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution
Article Abstract:
The molecular composition of a vinyl chloride (VC) reductase as well as the encoding gene was described using the highly enriched Dehalococcoides sp. strain VS. The results suggest that the enzyme described here is an environmentally relevant VC reductase and that molecular probing methods, based on the vcrA gene sequence, can be useful tools to assess the capacity for VC reduction at contaminated sites.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species
Article Abstract:
A computational analysis of the codon usage of the vinyl chloride reductases (VC-RDase) genes vcrA and bvcA has shown that these genes are highly unusual and are characterized by a low G+C fraction at the third position. The high level of abnormality in the codon usage of VC-RDase genes has shown an evolutionary history that is different from that of most other Dehalococcoides genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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