In vivo Himar1-based transposon mutagenesis of Francisella tularensis
Article Abstract:
The utilization of a conditionally replicating derivative for delivery of a modified Himar1 (HimarFT) into Francisella tularensis live vaccine strain (LVS) in vivo is reported. The results suggest that a comprehensive library of mutants could be generated in Francisella tularensis LVS, providing an additional genetic tool to identify virulence determinants required for survival within the host.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Discrimination between Francisella tularensis and Francisella-like endosymbionts when screening ticks by PCR
Article Abstract:
A study is conducted to show that tick pools initially positive for Francisella tularensis based on a 16S rRNA gene PCR assay are determined to be positive only for Francisella-like endosymbionts. The need for careful evaluation of PCR-based diagnostic in laboratories that screen ticks for Francisella tularensis is reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Methods of enhanced culture recovery of francisella tularensis
Article Abstract:
Improved recovery times and a sensitivity of 90 percent of francisella tularensis is demonstrated when cultures are inoculated using fresh tissues. Here, comparisons are also performed using samples from a tularemia outbreak in prairie dogs.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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