Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070
Article Abstract:
The benzoate dioxygenase genes from the gram-positive bacterium Rhodococcus sp. strain 19070 were expressed in E. coli and the resulting enzyme was characterized. Rhodococcus can use many hydrocarbons and aromatic compounds as its sole carbon source, which makes it a useful organism for bioremediation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Induction of bphA, encoding biphenyl dioxygenase, in two polychlorinated biphenyl-degrading bacteria, psychrotolerant Pseudomonas strain Cam-1 and mesophilic Burkholderia strain LB400
Article Abstract:
The genes for biphenyl dioxygenase are regulated differently in different bacteria. For this reason, the optimal strategy for stimulating PCB degradation will depend on which PCB-degrading bacterium is present in soil.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Metabolism of chlorotoluenes by Burkholderia sp. strain PS12 and toluene dioxygenase of Pseudonomas putida F1: evidence for monooxygenation by toluene and chlorobenzene dioxygenases
Article Abstract:
The metabolism of 2-chloro and 3-chlorotoluene by Burkholderia sp. strain PS12 and Pseudomonas putida F1 was analyzed to determine the biochemical characteristics of the doixygenolytic pathway. The toluene dioxygenase of Pseudomonas putida F1 and the chlorobenzene dioxygenase of Burkholderia sp. PS12 strain catalyzed the conversion of 2-, 3- and 4-chlorotoluene to dihydrodiols. The synthetic process was characterized by the formation of minor products such as methyl- and chloro-substituted 1,2-dihydroxycyclohexadienes from 2- and 3-chlorotoluene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1
- Abstracts: Degradation of substituted phenylurea herbicides by Arthrobacter globiformis strain D47 and characterization of a plasmid-associated hydrolase gene, puhA
- Abstracts: Quantitative detection of the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA, narG, nirK, and nosZ genes in soils
- Abstracts: Identification of genes involved in the activation of the Bacillus thuringiensis inhA metalloprotease gene at the onset of sporulation
- Abstracts: Critical factors influencing the occurrence of Vibrio cholerae in the environment of Bangladesh. Genotypes associated with virulence in environmental isolates of Vibrio cholerae