Inference of horizontal genetic transfer from molecular data: an approach using the bootstrap
Article Abstract:
A test for the occurence of horizontal gene transfer resulting in inconsistent nucleic acid sequences between taxa was developed using the bootstrap procedure. The method could detect nucleic acid inconsistencies without going through the construction of phylogenetic trees. The procedure was used to analyze the Drosophila Adh locus and the mariner transposable element, where previous analyses have not shown significance in their nucleic acid sequence differences. The results showed that using the bootstrap, a high significance could be assigned to a postulated horizontal transfer between the ancestors of Drosophila and Zaprionus.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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The evolution of insertion sequences within enteric bacteria
Article Abstract:
The nucleotide sequences of different isoforms of the insertion sequences IS1 IS3 and IS30 from Escherichia coli and other enteric bacteria were compared. In E. coli, sequence data showed high conservation, indicating that along the clonal lineages of a species, transposons have a high turnover and transfer rate. Such high levels of conservation were not observed when comparing sequences of other enteric bacteria. However, sequence analysis indicates that some forms of recombination events and horizontal transfer occured during the evolution of these sequences among diverse bacterial species.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences
Article Abstract:
The presence of insertion sequences (IS) or transposable elements in Escherichia coli and Salmonella typhimurium were surveyed by Southern blot analysis and polymerase chain reaction. IS1 and IS3, which were origially recovered from E. coli, were found in majority of the salmonellae studied while IS200, which is Salmonella-specific, was found in about 20% of E. coli strains tested. IS200, on the other hand, may be found in the common ancestor of the two species since it was found to be polymorphic in E. coli but homogenous in S. typhimurium.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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