Temperature and structural effects on transfer of double-stranded RNA among isolates of the chestnut blight fungus (Cryphonectria parasitica)
Article Abstract:
The hypovirulence phenotype in Cryphonectria parasitica, the causalagent of chestnut blight, is associated with a cytoplasmic, double-stranded RNA(dsRNA) virus. Factors which may restrict the transfer of this dsRNA were studied. The findings indicate that dsRNA can be transferred among compatible and incompatible isolates of C. parasitica. However, this transfer is limited due to the antagonistic interactions among the incompatible isolates. Furthermore, transfer occurs only through substrate hyphae. Temperature is alsoan important factor limiting transfer. Freezing temperatures cause a loss of the dsRNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
DNA fingerprinting and analysis of population structure in the chestnut blight fungus, Cryphonectria parasitica
Article Abstract:
A dispersed, moderately repetitive sequence in Cryphonectria parasistica, thecausal agent of chestnut blight, was cloned in pMS5 and used for DNA fingerprinting studies. DNA fingerprint analysis showed that pMS5 detected two linked fragments. Hybridization patterns did not change during vegetative growth or during sporulation. The results showed that the C. parasitica population is partly clonal in structure, with 6 of the 33 fingerprint genotypes occurring twice. However, a large portion of the individuals in the population result from sexual reproduction.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica
Article Abstract:
The gene enpg-1 from the chestnut blight fungus, Cryphonectria parasitica that codes the main extracellular endopolygalacturonase (endoPG) does not contribute to fungal virulence. The gene product, ENPG-1 has a molecular weight of 34.5 kDa and a pI of 7.2, and is 66% similar to endoPG from the maize pathogen Cochliobolus carbonum. Isoelectric focusing of the polygalacturonase activity indicates that infected tissues have low ENGP-1 activity and disruption mutant-infected tissues have no ENGP-1.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: MCE activities and malathion resistances in field populations of the Australian sheep blowfly (Lucilia cuprina)
- Abstracts: Influence of water temperature and salinity on Vibrio vulnificus in Northern Gulf and Atlantic Coast oysters (Crassostrea virginica)
- Abstracts: Temperature and pH conditions that prevail during fermentation of sausages are optimal for production of the antilisterial bacteriocin sakacin K
- Abstracts: Evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from bovine rumen
- Abstracts: Characterization of transcription initiation and termination signals of the proteinase genes of Lactococcus lactis Wg2 and enhancement of proteolysis in L. lactis