Inhibition and facilitation of nucleic acid amplification

Article Abstract:

The wide application of DNA amplification technologies depends on the development of inexpensive, enhanced and automated detection and amplification systems. Current nucleic acid extraction approaches, however, indicate the difficulty of creating a single method which applies to all sample types. Available rapid-extraction methodologies employ sorption, size exclusion and ion-exchange chromatography.

Equipment and supplies, Chromosome mapping, Nucleic acids, Nucleic acid isolation

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Use of the IUL countermat automatic colony counter for spiral plated total viable counts

Article Abstract:

Use of automatic counters could allow large time savings when conducting spiral plated total viable counts. Using the spiral plate method with a wide range of foods and colony types, the IUL Countermat automatic colony counter was able to match total viable count results obtained from conventional manual counts.

Usage, Innovations, Product development, Counters (Electronics), Counters (Circuits), Plate counts (Microbiology), Feasibility studies

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Viability and DNA maintenance in nonculturable spiral Campylobacter jejuni cells after long-term exposure to low temperatures

Article Abstract:

Research was conducted to determine survival of common food-borne pathogenic bacteria at cold and warm temperatures. The data indicate that the bacterial cells survived upto 7 months at 4(sup)0 C as opposed to 15 days at 20(sup)0 C.

Spain, Methods, Testing, Observations, Campylobacter, Foodborne diseases, Cell culture, Tissue culture, Transmission electron microscopes

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