Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection
Article Abstract:
Interaction between the U1 snRNP and nonconserved intronic sequences has a role in 5' splice site selection. Intron definition and splice site selection take place early in assembly of the spliceosome, the complex that mediates pre-mRNA spicing. The yeast U1 snRNP-specific protein Nam8p is a component of the commitment complexes, the first stable complexes assembled on pre-mRNA. In vitro and in vivo, Nam8p becomes indispensable for efficient 5' splice site recognition when the process is inhibited by the presence of noncanonical 5' splice sites or absence of a cap structure. Nam8p is involved in a novel mechanism by which a snRNP component can affect splice site choice and regulate intron removal through its interaction with a nonconserved sequence.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
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3' splice site recognition in S. cerevisiae does not require base pairing with U1 snRNA
Article Abstract:
The role of the U1 small nuclear RNA (snRNA) in pre-mRNA splice site recognition in Saccharomyces cerevisiae was investigated. It had been proposed that the conserved nucleotides 9 and 10 of U1 snRNA engage in base pairing interactions with either the 5' exon or the 3' splice site sequences. Mutation studies showed that the U1 snRNA nucleotides at position 9 and 10 are involved in 5' splice site selection via their interaction with exon sequences, but are not essential for 3' splice site selection.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Mutations in U1 snRNA bypass the requirement for a cell type-specific RNA splicing factor
Article Abstract:
MER2 gene of Saccharaomyces cerevisiae is transcribed in meiotic and mitotic cells and undergo cell-type specific RNA splicing. It has been shown that efficient splicing of the primary transcript of MER2 requires the MER1 protein, which is produced by meiotic cells. Genetic mutation and selection shows that a mutation in SNR19, the gene for U1 snRNA, allows bypass of the MER1 requirement in MER2 RNA splicing.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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