Isoenzyme multiplicity and characterization of recombinant manganese peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium
Article Abstract:
A recombinant form of the manganese peroxidase from Phanerochaete chrysosporium can oxidize o-dianisidine and p-anisidine without externally added manganese. The enzyme was expressed in Aspergillus nidulans under the control of the alpha-amylase promoter from Aspergillus oryzae.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Heterologous expression of laccase cDNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns
Article Abstract:
Research has been conducted on Ceriporiopsis subvermispora homokaryon strains. The authors report that genomic clones which encode a putative laccase in these strains have led to allelic variant of lcs-1 gene, and that cDNA clone which corresponds to this gene has been expresed in Aspergillus nidulans and in A. niger, which supports the hypothesis that C. subvermispora has a single laccase gene and that isoforms result from post-translational process.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2003
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lip-Like genes in Phanerochaete sordida and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity
Article Abstract:
Two white rot fungi, Phanerochaete sordida and Ceriporiopsis subvermispora, contain lignin peroxidase (LiP)-like sequences but show no LiP activity. The LiP-like genes were identified by PCR amplification of the P. sordida and C. subvermispora genomes. Reverse transcription-PCR shows that the LiP-like gene of P. sordida is transcribed under ligninolytic conditions. However, the transcript is different from the genomic clone. There is no transcription of the C. subvermispora LiP-like gene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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