Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum
Article Abstract:
The proline dehydrogenase gene (putA) from the nitrogen-fixing bacteria Bradyrhizobium japonicum contains an open reading frame that codes for 1,016 amino acids. The putA gene encodes a protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activities like the Escherichia coli and Salmonella typhimurium genes. The B. japonicum putA gene lacks the C-terminal region and is not a part of the operon with proline porter gene unlike the E. coli and S. typhimurium genes. The protein contains the proline dehydrogenase and P5C dehydrogenase catalytic regions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Ammonia inhibition of plasmid pRmeGR4a conjugal transfer between Rhizobium meliloti strains
Article Abstract:
Ammonia inhibits plasmid conjugation between Rhizobium meliloti strains. A study of the effect of nutritional factors on two nonsymbiotic plasmids pRmeGR4a and pRmeGR4b of R. meliloti strains indicates that glutamate promotes transconjugation. The conjugation is reduced by a factor of 100 in media with glutamate and ammonium as nitrogen sources, in comparison with media containing only glutamate. The inhibition of conjugal transfer between R. meliloti strains by ammonia is however, unobservable in heterologous plasmids.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Carbon monoxide dehydrogenase activity in Bradyrhizobium japonicum
Article Abstract:
Research has been conducted on the Bradyrhizobium japonicum strain. The effect of carbon monoxide on the chemolithoautotrophic growth of this strain has been investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Molecular cloning, sequencing, and expression of a chemoreceptor gene from Leptospirillum ferrooxidans. Copper ions stimulate polyphosphate degradation and phosphate efflux in Acidithiobacillus ferrooxidans
- Abstracts: Methods of analysis and resources available for genetic trait mapping. A marker set for construction of a genetic map of the silver fox (Vulpes vulpes)
- Abstracts: Substrate selectivity and biochemical properties of 4-hydroxy- 2-keto-pentanoic acid aldolase from Escherichia coli
- Abstracts: Heavy-metal stress and developmental patterns of arbuscular mycorrhizal fungi. Effects of metal phytoextraction practices on the indigenous community of arbuscular mycorrhizal fungi at a metal-contaminated landfill
- Abstracts: Contamination of river water by Cryptosporidium parvum oocysts in western Japan. Detection of infectious Cryptosporidium parvum oocysts in mussels (Mytilus galloprovincialis) and cockles (Cerastoderma edule)