Isolation and entomotoxic properties of the Xenorhabdus nematophilus F1 lecithinase
Article Abstract:
Xenorhabdus nematophilus was used to characterize the pathological and biochemical nature of the lipases of entomopathogenic nematodes. Although phase I variants of X. nematophilus produced lecithinase after 16- to 24-hour incubation at 28 degrees centigrade, phase II did not produce any enzymatic activity even after several days. The isolates exhibited similar properties such as stability after exposure to purified lecithinase and narrow absorption spectra. Moreover, cytolytic activities with sheep erythrocytes or insect hematocytes were not observed with the five isolates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Conditional suicide system of Escherichia coli released into soil that uses the Bacillus subtilis sacB gene
Article Abstract:
The Bacillus subtilis sacB gene, which confers sucrose sensitivity upon gram-negative bacteria, was used as a conditional suicide system for Escherichia coli released into soil. The nptI-sacR-B cassette was integrated into the E. coli chromosome by using a nonreplicating circular vector. The effectiveness of the suicide system was then tested in soil microcosms. It was demonstrated that the suicide cassette has the ability to restrict the growth and survival of E. coli strains introduced into soil.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis
Article Abstract:
Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. An alternative to gene shuffling based on plasmid recombination is tested that reveals that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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- Abstracts: Diversity and heterogeneity of epibiotic bacterial communities on the marine nematode Eubostrichus dianae. Rapid and simple method for the most-probable-number estimation of arsenic-reducing bacteria
- Abstracts: Molecular and genetic analysis of the toxic effect of RAP1 overexpression in yeast. The clr1 locus regulates the expression of the cryptic mating-type loci of fission yeast
- Abstracts: Conservation status and habitat preferences of the Cozumel curassow. Habitat use by three breeding insectivorous birds in declining maple forests
- Abstracts: A soil and rhizosphere microorganism isolation and enumeration medium that inhibits Bacillus mycoides. A method for detection of pseudobactin, the siderophore produced by a plant-growth-promoting Pseudomonas strain, in the barley rhizosphere
- Abstracts: Distribution and abundance of Piping Plovers: results and implications of the 1991 International Census. Habitat-related factors affecting the distribution of nonbreeding American Avocets in coastal South Carolina