Molecular and genetic analysis of the toxic effect of RAP1 overexpression in yeast
Article Abstract:
The effects of RAP1 overexpression on the function of Rap1p (coded by RAP1) in the activation of ribosomal protein and glycolytic genes in yeast were investigated. Point mutations on RAP1 showed that overexpression of the gene produce a toxic effect. Electrophoretic mobility shift assays showed that small deletions within RAP1 coding sequences had no effect on toxicity. However, larger deletions in C-terminal sequences which infringe upon the DNA-binding domain suppressed the toxic effect, suggesting that the DNA-binding domain is required for toxicity. An adjacent region of unknown function is also suggested to play a role in relieving the toxic effect of RAP1 overexpression.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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The clr1 locus regulates the expression of the cryptic mating-type loci of fission yeast
Article Abstract:
The mat2 and mat3 loci of Schizosaccharomyces pombe aretranscriptionally silent genes which are involved in the determination of mating types in this yeast. Between these genes is the 'cold spot' for meioticrecombination. Six mutations were found which could allow expression of mat2 and mat3. These mutations were identified to a locus designated as clr1. In addition to gene expression, this locus also allowed meiotic recombination in the mat2-mat3 interval. These results indicate that transcriptional and recombinational repression proceed, at least in part, through the same mechanism.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Mutations in MGI genes convert Kluyveromyces lactis into a petite-positive yeast
Article Abstract:
The petite-negative yeast Kluyveromyces lactis was found to grow fermentatively in the absence of cytochrome c-mediated respiration after targeted disruption of the cytochrome c gene (CYC1). Three of four mitochondrial genome mutants isolated showed that nuclear mutations at two different loci, named mgi1 and mgi2, produce petites spontaneously due to deletions in mitochondrial DNA (mtDNA). Treatment with ethidium bromide likewise resulted in mtDNA deletions. Thus, the mgi gene products may be required for the integrity of the mtDNA.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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