Isolation, nucleotide sequence, and physiological relevance of the gene encoding triose phosphate isomerase from Kluyveromyces lactis

Article Abstract:

The cloning and sequencing of the Kluyveromyces lactis gene encoding triose phosphate isomerase activity (TIM) are described. The K lactic mutant strain, unlike Saccharomyces cerevisiae, was observed to have the ability to grow on glucose. Results of preliminary experiments of bioconversion suggest that, similar to the S cerevisiae TIM-deficient strain, the K lactis TIM-deficient strain is able to produce glycerol with high yield.

Genetic aspects, Phosphates, Nucleotide sequence, Base sequence, Bacterial genetics, Isomerases, Saccharomyces cerevisiae

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Efficient homolactic fermentation by Kluyveromyces lactis strains defective in pyruvate utilization and transformed with the heterologous LDH gene

Article Abstract:

Researchers have achieved a high yield of lactic acid per gram of glucose consumed by Kluyveromyces lactis that is devoid of pyruvate dehydrogenase and pyruvate decarboxylase activity but has high levels of lactate dehydrogenase activity. In addition, this technique produces few additional metabolites, which is one of the goals of industrial fermentation.

Fermentation, Lactic acid, Industrial microorganisms

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Inducible amplification of gene copy number and heterologous protein production in the yeast Kluyveromyces lactis

Article Abstract:

Research is conducted on the construction of the yeast Kluyveromyces lactis strain that contains a chromosomal recombinase A gene copy. Results demonstrate that copy number amplification increases the production of heterologous protein genes.

International, Physiological aspects, Proteins, Yeast, Yeast (Food product), Biotechnology, Gene amplification

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