Unstable expression and thermal instability of a species-specific cell surface epitope associated with a 66-kilodalton antigen recognized by monoclonal antibody EM-7G1 within serotypes of Listeria monocytogenes grown in nonselective and selective broths
Article Abstract:
The monoclonal antibody EM-7G1 was used in an enzyme-linked immunosorbent assay to identify conditions responsible for the unstable expression and heat instability of a cell surface epitope associated with a 66-kDa antigen in Listeria monocytogenes serotypes. The epitope was not present in serotypes 3b, 4a and 4c, which did not react with EM-7G1. However, the latter detected the 10 other serotypes when cells were grown in nonselective brain heart infusion broth or selective Listeria enrichment broth. EM-7G1 six of the 13 serotypes when cells were grown in repair broth while serogroup 4 could not be recognized.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Monoclonal antibody specific for Listeria monocytogenes associated with a 66kilodalton cell surface antigen
Article Abstract:
A monoclonal antibody (MAb) designated EM-7G1 was developed from a previously developed MAb which reacts to the 76-, 66-, 56- and 52-kDa antigens of Listeria monocytogenes. EM-7G1 reacts specifically to the 66-kDa antigen, and detected all strains of L. monocytogenes tested with no cross-reaction with other Listeria spp. Therefore,this MAb should be useful for detecting L. monocytogenes in food or clinical samples through enzyme-linked immunoassays or microcolony immunoblotting.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Monoclonal antibody-colony immunoblot method specific for isolation of Pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production
Article Abstract:
A monoclonal antibody (MAb) specific for the isolation of Pediococcus acidilactici (Ped-2B2) was developed and characterized. The MAb Ped-2B2 reacted with the P. acidilactici 116-kDa surface protein. The MAb probe was used in colony immunoblots to identify Pediococcus species from fermented foods. Biochemical characterization showed that all of the isolates were P. acidilactici that produce heat stable and proteinaceous bacteriocins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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