Katp, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli 0157:H7
Article Abstract:
The KatP gene which mediates the catalase-peroxidase activity of the enterhaemorrhagic Escherichia coli p0157 plasmid was sequenced to determine its molecular structure. The gene was located in the 9.7 kb SmaI deoxyribonucleic acid fragment. Nucleotide sequencing indicated the presence of peroxidase 1 and 2 motifs in the 736 amino acid polypeptide gene. Furthermore, open reading frames were also detected in the 2208 bp of the 81.8 kDa gene which is also homologous to bacterial bifunctional catalase-peroxidase genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
Article Abstract:
A study was conducted to analyze whether plasmids of Shiga-toxin-producing Escherichia coli (STEC) are a uniform genetic element present in STEC. Diarrhoeagenic E coli strains were examined using an espP-specific polymerase chain reaction. Results indicated differences in the espP genes of the STEC serotypes. Findings also showed that the plasmid of sorbitol-fermenting STEC 0157:H- did not have the espP and katP genes even though the genes were present in the plasmid of the non-sorbitol-fermenting STEC 0157:H7.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
Article Abstract:
The pO157 plasmid from enterohemorrhagic Escherichia coli (EHEC) O157:H7 was characterized at the molecular level. A restriction map was developed from seven restriction enzymes. The size of pO157 was found to be 93.6 kb and two additional genes were identified and designated as EHEC hlyB and EHEC hlyD. The hlyA, hlyB, hlyC and hlyD genes constitute a typical RTX determinant with the gene order CABD. An open reading frame was found adjacent to the EHEC hly operon.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Analysis of the effect exerted by extracellular pH on the maltose regulon in Escherichia coli K-12. Escherichia coli RNase II: characterization of the promoters involved in the transcription of rnb
- Abstracts: Construction of luciferase reporter bacteriophage A511::luxAB for rapid and sensitive detection of viable Listeria cells
- Abstracts: Deflocculation of activated sludge by the dissimilatory Fe(III)-reducing bacterium Shewanella alga BrY. Morphological and metabolic responses to starvation by the dissimilatory metal-reducing bacterium Shewanella alga BrY
- Abstracts: Initial steps in the degradation of methoxychlor by the white rot fungus Phanerochaete chrysosporium. Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaete laevis HHB-1625 and its extracellular ligninolytic enzymes
- Abstracts: Photosynthesis gene expression in Rhodobacter sphaeroides is regulated by redox changes which are linked to electron transport