Stress-induced transcriptional activation

Article Abstract:

Transcriptional activation induced by stress may be broken down into specific and general stress responses. Cells respond to heat stress by using protein folding and turnover mechanisms to protect cellular components. But there is also a general metabolic response to heat that is similar to that induced by oxidative stress, osmostress, or lack of essential nutrients. Specific transcription factors and promoter elements are involved in the changes in gene expression that underlie these reponses.

Analysis, Growth factors, Cellular signal transduction, Cell metabolism

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High-osmolarity signalling in Saccharomyces cerevisiae is modulated in carbon-source-dependent fashion

Article Abstract:

A research on the kinetics of HSP12 protein expression in various mutants was conducted to probe the role of high-osmolarity glycerol (HOG) and protein kinase A. (PKA) To determine the role of PKA activity in stress-induced signal transduction, the high-osmolarity response in yeast cells was examined through RNA isolation and Western blotting. Findings showed that the activity of PKA and the HOG pathway control the kinetics of HSP12 expression after high-osmolarity stress.

Usage, Protein kinases, Glycerol, Western immunoblotting, Western blot

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Double-stranded RNA viruses of Saccharomyces cerevisiae

Article Abstract:

Saccharomyces cerevisiae has the L-A virus which contains a double-stranded RNA for its genetic material. It is assumed that the double-stranded character of the virus' genetic material is to take advantage of the frameshift translation found in S. cerevisiae to produce varied products needed by the virus. The RNA was found to code for a toxin that was produced as a 'preprotein'.

Protein synthesis, RNA, Protein biosynthesis, Viral genetics

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