Maturation pathways for E. coli tRNA precursors: a random multienzyme process in vivo

Article Abstract:

Previous research has shown that maturation of transfer RNA in Escherichia coli occurs when the precursor sequences from both the 5' and 3' termini of an initial RNA transcript is removed. However, how this process works remains largely unknown. Using Northern analysis of RNA from Escherichia coli strains deficient in RNase, the processing intermediates that accumulate in the absence of processing nucleases are identified. These indicate that tRNA maturation is a stochastic process with no defined order and that can proceed with various alternative 3' processing nucleases.

Escherichia coli, Cellular signal transduction

---

Another bridge between kingdoms: tRNA splicing in archaea and eukaryotes

Article Abstract:

Transfer RNA (tRNA) splicing in archaea and eukaryotes connects the domains or kingdoms in the Woesian tree of life. Half-molecules are covalently connected by the tRNA ligase in a multistep approach involving GTP and adenosine triphosphate in pre-tRNA intron splicing. The 2' phosphate left at the splice junction is removed by phototransferase and is transferred to NAD. The process leads to the creation of a mature tRNA with a standard ligation junction linkage of 3'-5'.

Phylogeny, RNA splicing

---

The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors

Article Abstract:

The effects of La protein on yeast (S.cerevisiae) cell tRNA maturation was analyzed by using Northern blot analysis. La homologue protein 1 (Lhp1) stabilizes pre-tRNA in conformations that permits 3' end maturation to occur, by protecting the trailer end from exonucleases thus, ensuring an endonucleolytic cleavage, by recruiting endonucleases and directing them to the pre-tRNA site of cleavage.

Mutation (Biology), Mutation, Yeast fungi, Yeasts (Fungi), DNA polymerases

Similar abstracts:

• Abstracts: Redox regulation facilitates optimal peptide selection by MHC class I during antigen processing. Mechanisms of conformational change for a replicative hexameric helicase of SV40 large tumor antigen
• Abstracts: Mutational mechanisms for generating microsatellite allele-frequency distributions: an analysis of 4,558 markers
• Abstracts: An expectation-maximization algorithm for the analysis of allelic expression imbalance. Elevated expression and genetic association links the SOCS3 gene to atopic dermatitis
• Abstracts: Purification and partial characterization of a tripeptidase from Pediococcus pentosaceus K9.2. Development and characterization of lactose-positive Pediococcus species for milk fermentation
• Abstracts: Signal transduction via the multi-step phosphorelay: not necessarily a road less traveled. His-Asp phosphorelay: two components or more?

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.