Meiotic recombination on artificial chromosomes in yeast
Article Abstract:
The meiotic recombinations in artificial chromosomes composed mainly of bacteriophage lambda and minichromosome derivatives of Saccharomyces cerevisiae chromosome III were studied. The results showed that artificial chromosomes undergo very low frequencies of meiotic recombination. However, when a short fragment of yeast chromosome VIII was inserted within the right arm of the artificial chromosome, meiotic recombination frequencies mimicked the wild type levels of the short fragment. These findings were similar to those observed in the minichromosome experiments. These results indicate that meiotic recombination is regulated locally, rather than from afar.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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SID1-1: a mutation affecting meiotic sister-chromatid association in yeast
Article Abstract:
Errors in meiosis I were studied to detect diploids that give rise to disomes. A characterization of dominant allele SID1-1 was presented. Quantitation of meiotic segregation was done using the tetrad plating technique. Results showed that the SID1-1 effect does not cause any alteration in chromosome structure but decreased the incidence of complementary choromosome segregation in mitosis. Three types of segregational errors occurred in SID1-1 diploids and incidence increased in the wild types. SID1-1 errors were related to defective sister-chromatid association.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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Effects of homology, size and exchange on the meiotic segregation of model chromosomes in Saccharomyces cerevisiae
Article Abstract:
A model for analyzing the segregation of homologous chromosomes undergoing phase I meiosis has been developed. The model was tested using DNA sequences from Saccharomyces cerevisiae. Specifically, it was used to determine which DNA sequences moved away from each other during meiosis and to identify possible relationships between movement and chromosome size, recombination initiation and crossing over. Results showed that chromosome size and recombination initiation had negligible influence on the segregation of noncrossover chromosomes.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1996
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