Melanin production by a filamentous soil fungus in response to copper and localization of copper sulfide by sulfide-silver staining
Article Abstract:
Melanin is produced in the hyphal cell wall of the fungus Gaeumannomyces graminis and an electron-dense layer is formed between the cell wall and the inner chitin layer if it is grown in a culture containing copper (Cu). This electron-dense layer is probably formed of dihydroxynaphthalene melanin and the melanin layer does not allow toxic metal ions to enter the cell. If the fungus is then put into a culture containing very little Cu, melanization of the hyphae become less. Electron microscopic studies show that the Cu is present in the cell wall in the form of Cu sulfide.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Regulation of the periplasmic (Fe) hydrogenase by ferrous iron in Desulfovibrio vulgaris (Hildenborough)
Article Abstract:
Iron regulation of the hydrogenase enzyme is discussed. Periplasmic (Fe) hydrogenase was derived from the sulfate-reducing bacterium Desulfovibrio vulgaris DSM 8303 and subjected to growth analyses. Enzyme derepression and increases in the specificactivity occurred during growh at 5ppm of iron. However, the enzyme was repressed at the presence of 100 ppm of ferrous iron. It was found that hydrogen and nitrogen gas culturing of cells causes the operation of a repression-derepression phenomenon with ferrous iron.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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ISD1, an insertion element from the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough: structure, transposition, and distribution
Article Abstract:
The presence of insertion element ISD1 in two copies in the genome of Desulfovibrio vulgaris Hildenborough. Research revealed a length of 1,200 bp with a pair of 44-bp imperfect inverted repeats at the ends, flanked by a direct repeat of the 4-bp target sequence, using cloning and sequencing of a transposed copy of ISD1. ISD1-A and ISD1-B were not flanked by direct repeats as evidenced by isolation and analysis of the chromosomal copies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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