Membrane adsorption with direct cell culture combined with reverse transcription-PCR as fast method for identifying enteroviruses from sewage
Article Abstract:
A new approach for the detection and identification of enterviruses concentrated and isolated form sewage is presented. Viruses were absorbed to cellulose nitrate membrane filters, cultured directly from the membrane filters by using the VIRADEN method, and identified by reverse transcription-PCR, followed by 5' untranslated region (5'-UTR) restriction fragment length polymorphism (RFLP) analysis and partial sequencing of the VP1 protein coding region.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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A protective immune response is generated in rainbow trout by an OompH-like surface antigen (P18) of Flavobacterium psychrophilum
Article Abstract:
An investigation of the surface characteristics of Flavobacterium psychrophilum that is an important pathogen of fish is conducted to enable in identifying a surface protein termed P18, by a two-step method. The purification and characterization of the immunogenic and protective 18-kDa outer membrane-associated OmpH-like protein is reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Antigenic characterization of the fish pathogen Flavobacterium psychrophilum
Article Abstract:
The potential vaccine targets of Flavobacterium psychrophilum are a 16 kDa lipopolysaccharide and a 20 kDa protein. This organism causes rainbow trout fry syndrome and bacterial cold water disease.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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