Membrane topology analysis of the Escherichia coli cytosine permease
Article Abstract:
The Escherichia coli cytosine permease (CodB) contains 12 membrane-spanning sequences and the amino- and carboxy-terminals are present in the cell cytoplasm. Membrane topology studies using CodB fused to alkaline phosphatase or beta-galactosidase (BG) indicate that the 2, 4, 6, 8, 10 and 12 hydrophilic sites face the periplasmic space. The 1, 3, 5, 7, 11 and 13 hydrophilic sites face the cytoplasm. The 192nd amino acid is present in the third periplasmic domain. Activities of some CodB-BG fused enzyme disagree with the membrane topology model.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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A reappraisal of the diversity and class distribution of aspartate transcarbamoylases in Gram-negative bacteria
Article Abstract:
The identification of a class A aspartate transcarbamoylase (ATCase) in organisms other than fluorescent pseudomonads, and a class C ATCase from a Gram-negative organism, shows the diversity and distribution of ATCase. Of the 6 class A ATCases, one is purified from Acinetobacter calcoacetius, 2 from bacteria outside the gamma-subgroup of the Proteobacteria, and one from the alpha-subgroup and Deinococcus radiophilus. Evolutionary implications are discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Single electron transfer by an extracellular laccase from the white-rot fungus Pleurotus ostreatus
Article Abstract:
EPR and absorption spectroscopy help characterize an extracellular laccase I from the white-rot fungus Pleurotus ostreatus. Two ionization groups function as the ligand of copper metal in the active site of the laccase I. Analysis of the action of laccase I on 3,5-dimethoxy-5-hydroxyacetophenone by EPR spectroscopy reveals the involvement of the phenoxy radical as an intermediate in the single electron transfer catalyzed by laccase I.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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