Membrane vesicles derived from Pseudomonas aeruginosa and Shigella flexneri can be integrated into the surfaces of other Gram-negative bacteria

Article Abstract:

Research was conducted to examine the integration of membrane vesicles (MVs) obtained from Pseudomonas aeruginosa dps89 and Shigella flexneri M90T into Salmonella typhi Ty21a, Salmonella enterica serovar Typhimurium aroA and Escherichia coli DH5-alpha. It was demonstrated through electron microscopy of preparations during the integration of vesicle antigens that the MVs rapidly fused with the outer membrane of the host strains. The method described provides a simple efficient means of introducing exogenous surface antigens into the outer membranes of Gram-negative bacteria for possible vaccine use.

Gram-negative bacteria, Salmonella, Pseudomonas aeruginosa, Bacterial cell walls, Shigella flexneri

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Partial characterization of a major autolysin from Mycobacterium phlei

Article Abstract:

A study was conducted to analyze the autolytic enzyme profiles of mycobacteia using SDS-PAGE zymography supporting mycobacterial peptidoglycan sacculi as a substrate. An autolysin was determined to homogeneity by DEAE-cellulose chromatography and preparative gel electrophoresis. Purification steps were performed at 4 degrees C on ice. Experimental results indicated that M. phlei expressed two distinct autolysins.

Enzymes, Mycobacterium

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