Metabolic pathways utilized by Phanerochaete chrysosporium for degradation of the cyclodiene pesticide endosulfan
Article Abstract:
The white rot fungus Phanerochaete chrysosporium metabolizes the pesticide endosulfan using oxidative and hydrolytic pathways which are distinct from each other. The cytochrome P-450 suppressor piperonyl butoxide inhibits the the oxidation of endosulfan and increases its hydrolysis. The fungus metabolizes endosulfan under both nutrient-rich and nutrient-deficient conditions. The main metabolic products are endosulfan sulfate, endosulfan diol, endosulfan hydroxyether and endosulfan dialdehyde.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chrysosporium
Article Abstract:
The splicing of introns in the genes for two exocellobiohydrolases produced by the fungus Phanerochaete chrysosporium appears to be dependent upon the substrate of the enzymes. In the presence of avicel, the genes cbhI.1 and cbhI.2, which code for the binding domains of the enzymes, produce both spliced and unspliced mRNA transcripts. However, in the presence of carboxymethyl cellulose, the genes produce mostly spliced mRNA transcripts.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Stable defined substrate for turbidimetric assay of endoxylanases
Article Abstract:
A substrate preparation procedure was developed to optimize the assay conditions for xylanase activity. The important features of the preparation were autoclaving and two centrifugations. In addition, a quick freeze and rapid thaw step followed by the addtion of 2.7% of PEG 6000 could increase the sensitivity of the assay. The procedure could be used even in the presence of other reducing compounds.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Use of filamentous cyanobacteria for biodegradation of organic pollutants. Metabolism of diethyl ether and cometabolism of methyl tert-butyl ether by a filamentous fungus, a Graphium sp
- Abstracts: A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences. Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66
- Abstracts: Bacillus thuringiensis HD-73 spores have surface-localized Cry1Ac toxin: physiological and pathogenic consequences