Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer

Article Abstract:

Microbial community composition related to benzene oxidation under in situ Fe(III)-reducing conditions in a petroleum-contaminated aquifer located in Bemidji, MN, was studied. Findings indicated that specific Geobacter spp are associated with the capacity for benzene degradation in the petroleum-contaminated aquifer. Evidence supporting this conclusion includes the significant rise in the number of Geobacteraceae within the zone of benzene degradation and specific enrichment of a tight phylogenetic cluster of Geobacter spp in the zone of benzene degradation that is not found in the uncontaminated sediments.

Environmental aspects, Benzene, Aquifers, Anaerobic bacteria, Microbial populations

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A novel PhosphorImager-based technique for monitoring the microbial reduction of technetium

Article Abstract:

A method using PhosphorImager and paper chromatography shows that the metal-reducing bacteria Shewanella putrefaciens and Geobacter metallireducens reduce soluble technetium (Tc) to insoluble forms. S. putrefaciens produces Tc(V), Tc(IV) and one unidentified Tc species while G. metallireducens produces Tc(V) and the unidentified species. G. metallireducens removes almost 70% of the Tc from the solution while S. putrefaciens is unable to remove any Tc. The different Tc species separated by paper chromatography are identified by PhosphorImager.

Usage, Observations, Technetium, Chromatography, Chromatographic analysis

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Brominated biphenyls prime extensive microbial reductive dehalogenation of Aroclor 1260 in Housatonic river sediment

Article Abstract:

The priming activities of 14 mono-, di- and tribrominated biphenyls in anaerobic microcosms of sediment from Woods Pond in Lenox, MA, were tested to determine whether they can activate or 'prime' anaerobic microorganisms in the river sediment to reductively dehalogenate the Aroclor 1260 residue. Most of the brominated biphenyls primed Process N or Process P dechlorination, thus demonstrating that compounds aside from polychlorinated biphenyls (PCB) have the ability to activate extensive PCB dechlorination in sediment.

River sediments, Polybrominated biphenyls, Bromine

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