Mode of action of lactococcin B, a thiol-activated bacteriocin from Lactococcus lactis
Article Abstract:
Changes in the membrane potential, acidity gradient and amino acid uptake and efflux in the cells of Lactococcus lactis due to a purified lactococcin B (LcnB) bacteriocin is discussed. Measurements of membrane potential and intracellular acidity were determined by tetraphenylphosphonium-selective electrode and spectrofluorometry, respectively. Results indicate the presence of LcnB immune cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae
Article Abstract:
A novel NADP-dependent branched-chain alcohol dehydrogenase was isolated from Saccharomyces cerevisiae var uvarum developed in an anaerobic environment. Characterization of the alcohol dehydrogenase reveals that it has a monomeric quaternary structure, a 5.9 pI and a 37 kDa molecular mass. Results indicate that the enzyme may be used in making food flavorings during alcoholic fermentation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Germination of Bacillus cereus spores is induced by germinants for differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine
Article Abstract:
The germination induction upon adhesion to caco-2 cells in 11 enterotoxigenic strains of Bacillus cereus was investigated. It was found that spores of the 11 strains of Bacillus cereus adhered with similar efficiencies to Caco-2 cells, Hep-2 cells did not trigger germination and spores of all strains displayed similar germination efficiencies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Role of calcium in activity and stability of the Lactococcus lactis cell envelope proteinase. Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase
- Abstracts: Conversion of phenylalanine to benzaldehyde initiated by an aminotransferase in Lactobacillus plantarum. Application of p-toluidine in chromogenic detection of catechol and protocatechuate, diphenolic intermediates in catabolism of aromatic compounds
- Abstracts: Determination of plasmid DNA concentration maintained by nonculturable Escherichia coli in marine microcosms. Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification
- Abstracts: Characterization of the chemical and antimicrobial properties of piscicolin 126, a bacteriocin produced by Carnobacterium piscicola JG126
- Abstracts: Effect of compatible solutes on survival of lactic acid bacteria subjected to drying. Metabolism of tetralin (1,2,3,4-tetrahydronapthalene) in Corynebacterium sp.strain C125