Molecular biological evidence for the occurrence of uncultured members of the actinomycete line of descent in different environments and geographical locations
Article Abstract:
Genomic DNA isolated from a peat bog sample from Northern Germany was amplified and cloned. Of the 262 16S rDNA clones generated, 37 were Gram-positive. Sequence analysis of these Gram-positive bacteria revealed regions corresponding to sequences of the members of order Actinomycetales. DNA blotting and hybridization with oligonucleotide probes showed that the peat clones consist of three major groups, two of which are related to Acidimicrobium ferrooxidans and 'Candidatus Microthrix parvicella.' Phylogenetic analysis confirmed these findings and indicated that the third group is Rubrobacter radiotolerans.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Clostridium paradoxum DSM 7308T contains multiple 16S rRNA genes with heterogeneous intervening sequences
Article Abstract:
Fifteen different sequences in variable region I of the 16S rDNA were found in the cloned 16S rRNA genes of Clostridium paradoxum DSM 7308T using sequence analysis. Most of the cloned genes had intervening sequences (IVSs), which ranged in length from 120-131 nt, and were found in the DNA obtained from single colonies of C. paradoxum. Sequence analysis of the 16S rRNA reverse transcriptase (RT)-PCR product and Northern hybridization showed the absence of IVSs in the mature rRNA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils
Article Abstract:
The clone DA079 and two other identical clones were found to belong to the class of Actinobacteria found in Drentse grassland soils. The clones affiliation to the actinobacterial group was determined through the analysis of their 16S rRNA and fingerprints. The 16S rRNA was amplified by RT-PCR using conserved primers for the domain bacteria's members. Fingerprints were derive by subsequent sequence-specific separation by temperature-gradient gel electrophoresis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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