Molecular cloning and duplication of the nematode sex-determining gene tra-1
Article Abstract:
The Caenorhabditis elegans autosomal sex-determining gene tra-1 was cloned by identifying linked transposon insertions, about 200-kb upstream from tra-1. A genomic walk was done covering 400 kb using YAC, cosmid and phage clones. A variety of mutant alleles were also used to provide preliminary molecular information about the organization of the tra-1 locus. A 14-kb deletion encompassing the 5' region of the gene was used, which confirmed the null phenotype. Another 22-kb tandem duplication, eDp24, creates two almost complete copies of tra-1 and appears to form negative autoregulation at this locus.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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Finding treasures in frozen cells: New centriole intermediates
Article Abstract:
A study report on C. elegans, which provide new details about the events and genes involved in duplication of the centrioles through linear pathways for centriole assembly, is presented.
Publication Name: BioEssays
Subject: Biological sciences
ISSN: 0265-9247
Year: 2007
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