Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa
Article Abstract:
The P59Nc gene codes for a 59 kDa polypeptide which composes the 8-10 nm filaments of Neurospora crassa. It has been proposed that the snowflake (sn) locus codes for the P59Nc gene. The DNA segment coding for this polypeptide was cloned and characterized. Southern blotting and restriction fragment length polymorphism analyses showed that a gene, designated cfp, for cytoplasmic filament protein, located on the right arm of chromosome VII, codes for P59Nc. Mutation analyses showed that this locus is distinct from the sn locus. These results indicate that mutations in the sn locus which produce abberant P59Nc do not affect the cfp gene.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Inactivation of genes encoding subunits of the peripheral and membrane arms of neurospora mitochondrial complex I and effects on enzyme assembly
Article Abstract:
A nuclear gene in linkage group I codes for the 12.3-kD subunit of the membrane arm of Neurospora mitochondrial complex I, while another gene in linkage group IV codes for the 29.9-kD subunit of the peripheral arm of the complex. The two proteins which determine complex I assembly and result in enzyme sub-complex collection, are not involved in fungal growth. The absence of the 12.3-kD subunit inhibits the ultimate assembly of the two arms of complex I, while the peripheral arm formation is prevented by the lack of the 29.9-kD subunit, indicating the involvement of the 29.9-kD protein in the early stages of the complex assembly.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Isolation of Neurospora crassa A mating type mutants by repeat induced point (RIP) mutation
Article Abstract:
Functional regions of the A mating type allele in the fungus Neurospora crassa were identified by the use of repeat induced point (RIP) mutations. In the mt A-1 open reading frame region, RIP mutations caused the isolation of mutants which were sterile and heterokaryon-compatible. On the other hand, in a region outside mt A-1, RIP mutations caused the isolation of mutants which though fertile, were inadequate in formulating ascospores.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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