Molecular cloning of the Corynebacterium glutamicum ('Brevibacterium lactofermentum' AJ12036) odhA gene encoding a novel type of 2-oxoglutarate dehydrogenase

Article Abstract:

The molecular cloning and sequence of the odhA gene encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex) of Corynebacterium glutamicum, a coryneform, Gram-positive bacterium widely used in the industrial production of amino acids, are reported. The odhA gene consists of 3771 bp and a molecular mass of 138 656 Da was predicted for the OdhA polypeptide. It appears to be monocistronic, judging from its 3*9 kb transcript and the presence of a rho-independent terminator-like structure. Its C-terminal region shows a strong sequence similarity to E1os from other organisms. The amino acid sequence of its N-terminal extension indicates that it might encode a novel bifunctional protein with E10 and E2o activities.

Analysis, Cloning, Dehydrogenases, Oxidoreductases, Amino acid sequence, Amino acid sequencing

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23Na NMR spectroscopy of free Na+ in the halotolerant bacterium Brevibacterium sp. and Escherichia coli

Article Abstract:

Measurement of internal Na+ levels in the halophilic archaeobacterium Brevibacterium sp., and non-halophilic Escherichia coli through 23Na NMR spectroscopy reveal that growth phase does not influence the internal Na+ levels, which remain constant after storage for five days at two degrees Celsius. Intracellular sodium level in Brevibacterium sp. increases with increasing extracellular NaCl levels, with or without yeast in the growth medium, while free Na+ accumulation in E. coli cells is higher. Both strains exhibit an inverse relationship between variations in Na+ levels and growth rate. Addition of osmoprotectants to NaCl does not alter the internal Na+ concentration in E. coli, while it is lowered in Brevibacterium sp.

Archaeabacteria, Sodium, Sodium (Chemical element), Archaea

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NMR analyses of compatible solutes in a halotolerant Brevibacterium sp

Article Abstract:

Brevibacterium sp. (strain JCM 6894) is a different species from other members of the genus Brevibacterium. This was concluded following nuclear magnetic resonance analyses of compatible solutes in a halotolerant Brevibacterium sp. Glutamate, glycine betaine and hydroxyproline were the main compatible solutes in the cells at the lower osmolarity of complex medium. Ectoine was the predominant compatible solute at >1*5 M NaCl or KCl. In cells grown in chemically defined medium with 0-0*5 M NaCl, trehalose and glutamate were the main solutes. In the presence of >1*5 M NaCl and in complex medium, both ectoine and hydroxyectoine became dominant. Other compatible solutes identified were gamma-aminobutyrate and proline.

Usage, Nuclear magnetic resonance, Osmosis

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