Molecular forensic profiling of Cryptosporidium species and genotypes in raw water
Article Abstract:
The method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples is developed. The method is based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes of Cryptosporidium in a single water sample.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Comparison of animal infectivity and nucleic acid staining for assessment of Cryptosporidium parvum viability in water
Article Abstract:
Staining Cryptosporidium parvum oocysts with a fluorescent dye can distinguish between those that are viable and those that are not. A study using the fluorogenic dyes SYTO-9 and SYTO-59 showed that oocysts that fluoresced strongly were not infective whereas those that did not fluoresce or only fluoresced weakly were infective.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining
Article Abstract:
Researchers describe a system that can distinguish viable bacterial cells from damaged bacterial cells by using permeant and impermeant fluorescent probes. A full or partial quenching of the permeant probe by the impermeant probe will occur if the cell is damaged and no quenching will occur if it is intact and viable.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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- Abstracts: Effect of pasteurization on infectivity of Cryptosporidium parvum oocysts in water and milk. In vitro interactions of Asian freshwater clam (Corbicula fluminea) hemocytes and Cryptosporidium parvum oocysts
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