Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells
Article Abstract:
The ovo locus of Drosophila melanogaster was cloned and analyzed. The DNA lesions causing three ovo loss-of-function mutations and three svb- ovo- double mutations were identified. The mutations cluster within a 7-kilobase (kb) region that is homologous with the female-specific, germline-dependent transcription unit for a 5-kb poly(A)+ RNA size class. A cloned 10-kb segment that includes the mutant cluster and the transcription unit for the 5-kb RNA size class without a neighboring 6-kb RNA transcription unit can reverse the germline effects of ovo mutations.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
The transposable element mariner mediates germline trsnsformation in Drosophila melanogaster
Article Abstract:
Researchers used the transportable element mariner to design a vector, called pMIwB, for germline transformation in Drosophila melanogaster. It has a mariner element disrupted by an insertion having the wild-type white gene from D. melanogaster, the beta-galactosidase gene from Escherichia coli and sequences that assist in the plasmid replication and selection in E. coli. The mariner transposable element can enhance the introduction of the big (13.2kb) MIwB element, hinting that mariner may offer a large host-range transformation vector for insects.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Identification of regions interacting with ovo(D) mutations: potential new genes involved in germline sex determination or differentiation in Drosophila melanogaster
Article Abstract:
Two regions in the Drosophila melanogaster germline are identified. Their gene products function in developmental hierarchies. The two regions consist of several germline sex determination genes. The presence of the two regions is identified by the development of new systematic screening techniques.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Molecular chaperones activate the Drosophila ecdysone receptor, an RXR heterodimer. Noncoding RNA genes in dosage compensation and imprinting
- Abstracts: The product of the yvoC (gerF) gene of Bacillus subtilis is required for spore germination. Transcriptional responses during outgrowth of Bacillus subtilis endospores
- Abstracts: Drosophila fasciclinll is required for the formation of odor memories and for normal sensitivity to alcohol. The EGF receptor defines domains of cell cycle prgression and survival to regulate cell number in the developing Drosophila eye
- Abstracts: The Drosophila couch potato gene: an essential gene required for normal adult behavior. P-element mutations affecting embryonic peripheral nervous system development in Drosophila melanogaster
- Abstracts: Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp. strain M. Isolation and characterization of cesium-accumulating bacteria