Molecular movement inside the translational engine

Article Abstract:

The translocation elongation cycle mechanism is characterized by the iterative coupled movement of mRNA and tRNA throughout the elongation phase of protein synthesis. The ribosome provides specific binding sites for mRNA, tRNA and the termination factors. It also stabilizes codon-anticodon interaction and preserves the translational reading frame. Moreover, RNAs bind in the various ribosomal binding states to protect rRNA characteristic bases from chemical probes and to provide structure correlates for the tRNA states during the elongation cycle.

Proteins, Protein synthesis, Translocation (Genetics), Transfer RNA

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mRNA helicase activity of the ribosome

Article Abstract:

Various evidences prove that the helicase activity resides within the ribosome itself. Using an in vitro translation system with purified components, the helicase activity is localized to the30S ribosomal subunit that suggests that ribosomal proteins S3, S4, and S5, which encircle the incoming mRNA, play a role in helicase processivity.

Science & research, Gene mutations, Gene mutation, Ribosomal proteins

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The path of messenger RNA through the ribosome

Article Abstract:

Examination of the path of mRNA through the 70S ribosome reveal that about 30 nucleotides are wrapped in a groove encircling the neck of the 30S subunit. The mRNA entry into the ribosome occurs at about +13 to +15 nucleotide position.

Statistical Data Included, Methods, Ribosomes, Molecular structure, X-ray crystallography, Fourier analysis

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