Molecular cloning and analysis of the ptsHI operon in Lactobacillus sake

Article Abstract:

The ptsH and ptsI genes of Lactobacillus sake, which encode the general enzymes of the phosphoenolpyruvate carbohydrate phosphotransferase system (PTS), were cloned and sequenced. Nucleotide sequence and mRNA analysis indicated that the two genes are cotranscribed in a large transcript encoding both HPr and enzyme I. The transcription of ptsHI was not affected by the carbon source. Four ptsI mutants were formed by single-crossover recombination and for all mutants, growth on PTS carbohydrates was eliminated.

Microbial enzymes, Transferases

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Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene to study regulation of the putative copper ATPase, AtkB

Article Abstract:

Research describes the construction of a Lactobacillus sakei strain containing an atkYB locus representing the genes atkY and atkB encoding AtkB and AtkY proteins, which show sequence similarity to copper P-type ATPase. Data from the transcriptional regulation of the atkYB operon reveal that the operon is induced by low concentration of copper sulfate.

United States, Analysis, Usage, Enzymes, Adenosine triphosphatase, Copper sulfate

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Characterization of a theta-type plasmid from Lactobacillus sakei: a potential basis for a low-copy-number vectors in lactobacilli

Article Abstract:

Research has been conducted on Lactobacillus sakei plasmid. The authors have analyzed the complete sequence of this plasmid, and have described its relationship with other plasmids, its replication characterization and its ability to be present in different lactobacilli.

Science & research, Biological Product (except Diagnostic) Manufacturing, Nucleic Acid Derivatives, Environmental aspects, Plasmids, Microbiology, Nucleotides, Microbial populations, Microbial ecology

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