Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments

Article Abstract:

Subsurface bacterial strains expressing a plasmid, containing either toluene dioxygenase gene (tod) or toluene-4-monooxygenase (tmo) gene, and either an Escherichia coli tac (Ptac) or a Pseudomonas putida meta (Pm) promotor degrade both toluene and trichloroethylene (TCE). The plasmids are introduced into the bacteria by electroporation. Toluene degradation is higher in strains expressing Ptac while TCE degradation is similar in the presence of both Ptac and Pm. The tod gene is more efficient for toluene degradation while tmo is better for TCE degradation.

Analysis, Physiological aspects, Observations, Microbial genetic engineering, Gene expression, Toluene, Trichloroethylene

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Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway

Article Abstract:

Research was conducted to examine the reductive dechlorination of tetrachloroethene to ethene by two novel dehalogenases acting as catalysts. The two membrane-bound dehalogenases underwent partial purification from an anaerobic mixed culture with Dehalococcoides ethenogenes. The light-reversible inhibition of PCE-RDase by iodoethane and the light-reversible inhibition of TCE-RDase by iodopropane indicate that both dehalogenases have Co(I) corrinoid cofactors. Results provide further insight into the catalytic mechanisms of reductive dehalogenation.

Bacteriology, Bacterial cultures

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Trichloroethene reductive dehalogenase from Dehalococcoides ethenogenes: sequence of tceA and substrate range characterization

Article Abstract:

The trichloroethene reductive dehalogenase from Dehalococcoides ethenogenes can dechlorinate tetrachloroethene, trichloroethene, dichloroethane and dibromoethane, resulting in ethene. This bacterium is the only known organism that can biodegrade tetrachloroethene and trichloroethene, which are common groundwater contaminants.

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