Monoclonal antibodies for detection of the H7 antigen of Escherichia coli
Article Abstract:
The murine monoclonal antibodies (MAbs), 2B7 and 46E9-99, are effective in the detection of the Escherichia coli O157:H7 antigen in food, with or without anti-O157-specific antibodies. The MAbs respond strongly to only E. Coli H7, H23 and H24 out of 216 whole-bacteria. The half purified H7 flagellin of E. coli O157:H7 is effective in binding the MAbs to the O157:H7 antigens. The response of a combination of polyconal antisera and MAbs to E. Coli H7 bacteria is very strong. The polyconal antisera, however, has a strong sensitivity to E. Coli without H7 and to bacteria without E. Coli.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Molecular serotyping of Escherichia coli O26:H11
Article Abstract:
A molecular serotyping protocol was developed to target the somatic and flagellar antigens of Escherichia coli O26:H11 used in traditional serotyping. It is concluded that Escherichia coli O26:H11 accurately determined O26 and H11 serotypes strains by traditional methods thus, affording primary laboratories serotyping capabilities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Isolation of Salmonella enterica and shiga-toxigenic Escherichia coli O157 from feces of animals in public contact areas of United States zoological parks
Article Abstract:
The shiga-toxigenic strains of Escherichia coli and Salmonella enterica were found to be the major cause of the zoonotonic enteric human diseases associated with the contact with animals. The infection risk was assessed by calculating the occurrence of the pathogens in animals from different zoos.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Amplified intergenic locus polymorphism as a basis for bacterial typing of Listeria spp. and Escherichia coli
- Abstracts: Dancing around the divisome: Asymmetric chromosome segregation in Escherichia coli. Homologous recombination and nonhomologous end-joining repair pathways regulate fragile site stability
- Abstracts: Two novel bacterial biosensors for detection of nitrate availability in the rhizosphere. Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation
- Abstracts: A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters
- Abstracts: New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities