Mutations in POL1 increase the mitotic instability of tandem inverted repeats in Saccharomyces cerevisiae
Article Abstract:
Synthetic long tandem inverted repeats (LTIRS) having 30 and 80 base pair-unit repeats are mitotically unstable when inserted in yeast due mainly to deletions that cause the complete of partial removal of the presumed hairpin structure. A minimum of one deletion endpoint is located close to or at the hairpin base and a majority of repaired junctions are found within short direct sequence repeats. Chromosomal position, inverted repeat length and repeat unit intervals were found to have an effect on the frequency of hairpin excision. POL1/CDC17 mutations were found to cause a significant increase in hairpin deletions.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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A novel mutation in DNA topoisomerase I of yeast causes DNA damage and RAD9-dependent cell cycle arrest
Article Abstract:
A missense mutation in the gene encoding toposiomerase I (TOP 1), which results in mitotic hypercombination, was isolated and described. The mutation labeled as top1-103 was also indicated by slow growth, constitutive expression of DNA damage-inducible genes and inviability in the absence of the double-strand break repair system. Results showed that the TOP1-103 enzyme directly damaged the DNA and caused RAD 9-dependent cell cycle arrest in G2 with overexpression of top1-13, similar to the action of wild-type TOP-1 in the presence of camptothecin.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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