N-Myristoylation of Arf proteins in Candida albicans: an in vivo assay for evaluating antifungal inhibitors of myristoyl-CoA: protein N-myristoyltransferase
Article Abstract:
An in vivo assay for N-myristoylation of Arf proteins in Candida albicans was developed to assess the efficacy of antifungal inhibitors of myristoyl-CoA. This protein mobility shift assay have shown that the myristoylated species migrated more rapidly than the nonmyristoylated species and 100% of the Arf is N-myristoylated in an NMT/nmt strain. A >50% reduction in Arf N-myristoylation appears to be a biochemical marker of a growth arrested cell. The antifungal activity of Sc-59383, a selective inhibitor of C.albicans Nmt was demonstrated by the mobility shift assay.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Oestrogen-binding protein in Candida albicans: antibody development and cellular localization by electron immunocytochemistry
Article Abstract:
The estrogen-binding protein (EBP) is usually situated close to the inner surface of the tonoplast membrane of the vacuole in Candida albicans, as observed by electron microscopy and antigen identification by immunogold staining. The purified EBP from C. albicans has a subunit molecular mass of about 46 kDa, as determined by the denaturing gel electrophoresis. The antibodies against EBP from Candida albicans are developed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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A DNA-binding protein from Candida albicans that binds to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence of C. albicans
Article Abstract:
A specific DNA-binding protein of Candida albicans called RPG-box-binding protein 1 (Rbf1p) was identified. This protein that binds to the Saccharomyces cerevisiae RPG boxes is a new transcription factor based on experimental results. It showed binding activity to the C. albicans telomeric repeat sequence. The purification of Rbf1p and the expression cloning of its encoding gene were described.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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