NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription

Article Abstract:

NPAT, a substrate of the cyclin E-Cdk2 complex, has been found to link cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. NPAT was shown to activate histone gene transcription, and the activation depends on promoter elements already thought to mediate cell cycle-dependent transcription.

Statistical Data Included, Physiological aspects, Histones, Eukaryotic cells, Cells (Biology), Eukaryotes, Genetic transcription, Transcription (Genetics)

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Nuclear organization of DNA replication in primary mammalian cells

Article Abstract:

Methods that conserve nuclear architecture have been used to analyze the spatial organization of the initiation of mammal DNA synthesis. Primary fibroblasts, it was found, start synthesis in a limited number of loci that have replication proteins. They surround the nucleolus and overlap already-identified internal lamin A/C structures. It appears that in normal mammal cells, the onset of DNA synthesis is coordinately regulated at a small number of previously not recognized perinucleolar sites selected in early G(sub.1)-phase.

DNA, Mammals, Cell nuclei, Cell nucleus, Fibroblasts, Retinoblastoma

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Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry

Article Abstract:

For understanding of mechanisms CDKs use to regulate cell cycle progression, identifying and characterizing the physiological substrates of the kinases is required. A screening method to identify novel CDK substrates has been found. A cDNA identified in the screen is the same as the recently isolated NPAT gene. NPAT works with cyclin E-CDK2 in vivo and can be phosphorylated by it. The protein level of NPAT is at its peak at the G1/S boundary. Overexpression of NPAT speeds S-phase entry. The effect is helped along by coexpression of cylin E-CDK2. It seems NPAT is a substrate of cyclin E-CDK2 and is a factor in S-phase entry.

Methods, Molecular biology

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