New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
Article Abstract:
Site-directed marker exchange mutagenesis in Xanthomonas campestris has been studied through two new plasmid vectors having a marker gene which can distinguish between single- and double-crossover events. The vectors contain the gusA marker gene which allow for the calorimetric screening of bacteria having these sequences. The gusA gene was originally isolated in Escherichia coli and is a popular marker used widely in studying plant gene expression. It encodes the beta-glucuronidase enzyme which is better than other reporter systems due to its simplicity and robustness.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Evaluation of the Biolog substrate utilization system to identify and assessmetabolic variation among strains of Xanthomonas campestris pv. Citri
Article Abstract:
The utilization of the Biolog substrate utilization system to obtain the metabolic fingerprints of 148 strains of Xanthomonas campestris pv. citri associated to various forms of citrus bacterial canker disease is discussed. The commercial Microlog 2N data base was unable to correctly identify the studied strains. However, by supplementingthe data base, there was a significant rise in the percentage of correct identifications. Results indicate that the utility of metabolic fingerprinting can be further enhanced by combining other methods with it.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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An oxidoreductase is involved in cercosporin degradation by the bacterium Xanthomonas campestris pv. zinniae
Article Abstract:
Five non-cercosporin-degrading mutants of a strain that rapidly degrades cercosporin (XCZ-3) were generated by ethyl methanesulfonate mutagenesis and were then transformed with a genomic library from the wild-type strain. Quantitative reverse transcription-PCR (RT-PCR) showed that the expression of both of these genes in the wild-type strain is upregulated after exposure to cercosporin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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